Fluorogenic protein labeling using a genetically encoded unstrained alkene† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc03635j Click here for additional data file.

α-CH acidity of alkyl–B(C6F5)2 compounds – the role of stabilized borata-alkene formation in frustrated Lewis pair chemistry† †Electronic supplementary information (ESI) available: Details of the DFT calculations and experimental details. CCDC 1006899–1006903. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c4sc01711k Click here for additional data file. Click here for additional data file.

Fluorogenic protein labeling using a genetically encoded unstrained alkene.

We developed a new fluorogenic bioorthogonal reaction that is based on the inverse electron-demand Diels-Alder reaction between styrene (an unstrained alkene) and a simple tetrazine. The reaction forms a new fluorophore with no literature precedent. We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a styrene-containing amino acid into...

ENDOR characterization of an iron–alkene complex provides insight into a corresponding organometallic intermediate of nitrogenase† †Electronic supplementary information (ESI) available: Additional spectroscopic and crystallographic information. CCDC 1543306. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc01602f Click here for additional data file. Click here for additional data file.

Dynamic multicolor protein labeling in living cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01364g Click here for additional data file. Click here for additional data file. Click here for additional data file.

Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc02842j Click here for additional data file.